Microsatellite marker identification using genome screening and restriction-ligation
نویسندگان
چکیده
منابع مشابه
Microsatellite marker identification using genome screening and restriction-ligation.
We have identified a fast and easy method for finding microsatellite markers that utilizes genome screening with inter-simple sequence repeat (ISSR) primers to detect microsatellite regions and to obtain sequence information flanking one side of the microsatellites and a restriction-ligation technique with a specific adaptor to allow sequence walking to obtain sequence information flanking the ...
متن کاملGenome-Wide Microsatellite Identification in the Fungus Anisogramma anomala Using Illumina Sequencing and Genome Assembly
High-throughput sequencing has been dramatically accelerating the discovery of microsatellite markers (also known as Simple Sequence Repeats). Both 454 and Illumina reads have been used directly in microsatellite discovery and primer design (the "Seq-to-SSR" approach). However, constraints of this approach include: 1) many microsatellite-containing reads do not have sufficient flanking sequence...
متن کاملPhylogenetic screening of a bacterial, metagenomic library using homing endonuclease restriction and marker insertion
Metagenomics provides access to the uncultured majority of the microbial world. The approaches employed in this field have, however, had limited success in linking functional genes to the taxonomic or phylogenetic origin of the organism they belong to. Here we present an efficient strategy to recover environmental DNA fragments that contain phylogenetic marker genes from metagenomic libraries. ...
متن کاملIdentification of a polymorphic Plasmodium vivax microsatellite marker.
Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite Plasmodium falciparum. Genetic markers for P. vivax would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a P. vivax telomeric clone that contai...
متن کاملHybrids in fishes and their identification using microsatellite DNA markers: A review
Among vertebrates, most of the fishes are unique for the lack of acrosome in their spermatozoa. The sperm enters the egg through micropyle of the egg. This has facilitated heterologous fertilization in many fishes and led to hybridization and polyploidization. Hybridization is performed both in the nature and domesticated fishes. Today, some of the hybrids have high growth rate than their paren...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2007
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112415